[phenixbb] what about heavy atom as model for MR

赵岩 zhaoy at moon.ibp.ac.cn
Sun Jun 23 06:52:39 PDT 2013


Hi everyone,

The heavy atoms(Se-Met) were located at P4322 space group(4A). But the initial
density map is so bad that I can not build the initial model. 

Meanwhile, I have another two datasets, native(2.6A) and  se-met anomalous
dataset with space group P2221. But these two datasets have no solution. I have
tried the initial map from sp P4322 as a model to do molecular  replacement.
But no MR solution. 

So, I want to know how can I use the heavy atom as model to do MR with
isomorphous or anomalous differences?

Thanks!

--

Yan Zhao, M.Phil.
National Laboratory of Protein Sciences 
Institute of Biophysics 
Chinese Academy of Sciences 
15 Datun Rd.
Beijing, 100101 
China 
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