[phenixbb] Alternate sequence
nwmoriarty at lbl.gov
Tue Aug 12 13:06:29 PDT 2014
Can you send me the model?
NB. Any files sent to me will be held in strictest confidence.
On Mon, Aug 11, 2014 at 10:28 AM, Mullins, Elwood A. <
elwood.mullins at vanderbilt.edu> wrote:
> Hello all,
> I'm having trouble refining a protein-DNA complex in which one of the DNA
> strands has a mixture of two different nucleotides at one position. Both
> are non-standard and require CIF files. I tried assigning each nucleotide
> the same chain identifier and the same residue number but unique alternate
> location identifiers. This didn't work. Phenix refined the model but only
> linked one of the nucleotides with the rest of the chain, pushing the 3' OH
> and 5' phosphate out of the density for the other nucleotide. Coot won't
> even open the model output by Phenix. I assume there's some way to do this.
> Thanks in advance,
> Elwood A. Mullins, Ph.D. <> elwood.mullins at vanderbilt.edu <> tel
> 615.936.5234 <> fax 615.343.6707
> phenixbb mailing list
> phenixbb at phenix-online.org
Nigel W. Moriarty
Building 64R0246B, Physical Biosciences Division
Lawrence Berkeley National Laboratory
Berkeley, CA 94720-8235
Phone : 510-486-5709 Email : NWMoriarty at LBL.gov
Fax : 510-486-5909 Web : CCI.LBL.gov
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