[phenixbb] Anomalous or not?
CPMAS Chen
cpmasmit at gmail.com
Thu Jul 10 10:29:08 PDT 2014
Phil, the Br is in ligand, as you suggest, I might just see weak anomalous.
Ryan, you are right. I have no evidence to show the Br-ligand I used is in
the protein structure.
resolution does not degrade much along with frames
I think this is just a weak anomalous signal datasets.
Thanks.
Charles
On Thu, Jul 10, 2014 at 1:03 PM, Phil Jeffrey <pjeffrey at princeton.edu>
wrote:
> Charles,
>
> So if you have bound ligand with a covalently-linked Bromine, and the
> ligand isn't abundant in your unit cell, don't you *expect* the anomalous
> signal to be weak ? Have you tried a DANO model-phased difference map or
> something comparable ?
>
> Phil Jeffrey
> Princeton
>
>
> On 7/10/14 12:52 PM, CPMAS Chen wrote:
>
>> Well, let me make this clear.
>>
>> 1. I am using Br anomalous signal to identify the potentially bound
>> ligand.
>> 2. I do shoot the crystals at 0.92A.
>> 3. the different crystals have different resolution, but anomalous
>> signal was weak as reported by autoxds.
>>
>> Charles
>>
>
>
>
--
***************************************************
Charles Chen
Research Associate
University of Pittsburgh School of Medicine
Department of Anesthesiology
******************************************************
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