[phenixbb] Iron (FE2) metalloprotein

Tim Gruene tg at shelx.uni-ac.gwdg.de
Tue Jun 24 04:01:21 PDT 2014

Hello Egor,

maybe radiation damage reduced the ion and it settled off? If you
collected at a wavelength were you have some anomalous signal, you
should check the anomalous difference map for a peak. That's one of the
most sensitive methods you can get. Try ANODE, for example.


On 06/24/2014 06:35 AM, Egor Tchesnokov wrote:
> Hello,
> Active site contains iron (FE2) coordinated by three histidines.
> After Phaser the active site does not contain un-explained green density.
> After Refinement blobs of various shapes appear in the vicinity  and upto 3-4A of active site iron.
> Refinement is done using occupancy and anomalous of iron options.
> Buffers do not contain molecules that could fit in those blobs.
> Any suggestions?
> Thank you
> Egor
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Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen


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