[phenixbb] any idea what is this? (unidentifiable blob in electron density map)

Tim Gruene tg at shelx.uni-ac.gwdg.de
Wed May 21 03:10:16 PDT 2014


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Dear,

you could try with a disordered acetate. The oxygen could interact
with the N in the Trp and the other O an C might flip positions.

Best,
Tim

On 05/21/2014 06:39 AM, Murpholino Peligro wrote:
> 1) 2FOFCWT PH2FOFCWT bue map (front1.png) (process: XDS->Aimless
> within CCP4i->MR with
> Phaser->Phenix.refine/coot->Phenix-refine-withwaters/coot) 2) FWT
> PHWT   purple map (front2.png) (Output from Aimless and calculate
> Fs with refmac within coot with last pdb from 1st line)
> 
> 
> 
> What do I have in the crystal? NaCl, Sodium acetate, water,
> lysozyme and some complexes (~ 400 daltons) that chelate Cu2+
> 
> Is there like a gallery of crazy-looking blobs that are explained
> with something ....real and possible?  (that would be great help
> for the newb)
> 
> Thanks in advance.
> 
> links to screenshots right here: 
> https://www.dropbox.com/s/rzjepvr8genolxi/front1.png 
> https://www.dropbox.com/s/0iycf4lh5y726x4/front2.png 
> https://www.dropbox.com/s/xx5kvgn91p763ei/faraway.png (1.0 rmsd for
> both maps above and 3.5 for the "red and green maps")
> 
> 
> 
> _______________________________________________ phenixbb mailing
> list phenixbb at phenix-online.org 
> http://phenix-online.org/mailman/listinfo/phenixbb
> 

- -- 
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

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