[phenixbb] any idea what is this? (unidentifiable blob in electron density map)
Tim Gruene
tg at shelx.uni-ac.gwdg.de
Wed May 21 03:10:16 PDT 2014
-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1
Dear,
you could try with a disordered acetate. The oxygen could interact
with the N in the Trp and the other O an C might flip positions.
Best,
Tim
On 05/21/2014 06:39 AM, Murpholino Peligro wrote:
> 1) 2FOFCWT PH2FOFCWT bue map (front1.png) (process: XDS->Aimless
> within CCP4i->MR with
> Phaser->Phenix.refine/coot->Phenix-refine-withwaters/coot) 2) FWT
> PHWT purple map (front2.png) (Output from Aimless and calculate
> Fs with refmac within coot with last pdb from 1st line)
>
>
>
> What do I have in the crystal? NaCl, Sodium acetate, water,
> lysozyme and some complexes (~ 400 daltons) that chelate Cu2+
>
> Is there like a gallery of crazy-looking blobs that are explained
> with something ....real and possible? (that would be great help
> for the newb)
>
> Thanks in advance.
>
> links to screenshots right here:
> https://www.dropbox.com/s/rzjepvr8genolxi/front1.png
> https://www.dropbox.com/s/0iycf4lh5y726x4/front2.png
> https://www.dropbox.com/s/xx5kvgn91p763ei/faraway.png (1.0 rmsd for
> both maps above and 3.5 for the "red and green maps")
>
>
>
> _______________________________________________ phenixbb mailing
> list phenixbb at phenix-online.org
> http://phenix-online.org/mailman/listinfo/phenixbb
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.4.12 (GNU/Linux)
Comment: Using GnuPG with Icedove - http://www.enigmail.net/
iD8DBQFTfHuIUxlJ7aRr7hoRAr57AJwPsQkvv5YrzQklXiMAM4gbIOopvQCfUdp4
95i7TPz/gdtcl1LRWvyZmmU=
=5rcV
-----END PGP SIGNATURE-----
More information about the phenixbb
mailing list