[phenixbb] anomalous signal

[Joao Dias]

Dear Matteo,
When you collect data at the Zinc K-edge, you will have a very small component of Co in the f'', since your protein as both Zn and Co.
On the other hand, the f' will still have a significant contribution of both Zn and Co.
I usually check Ethan's website and I am attaching a figure for the Zn, Co absorption edges:
http://skuld.bmsc.washington.edu/scatter/AS_form.html

Best regards,
Joao

Dr Joao Dias
Principal Scientist

Heptares Therapeutics Ltd
BioPark, Broadwater Road,
Welwyn Garden City,
Herts, AL7 3AX
UK

This document contains company confidential and/or proprietary information. It is intended for the exclusive attention of the addressee(s) above and should not be copied or disclosed to any other. If you have received this transmission in error, please make no use of its contents and contact the sender.



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-----Original Message-----
From: phenixbb-bounces at phenix-online.org [mailto:phenixbb-bounces at phenix-online.org] On Behalf Of Matteo Colombo
Sent: 20 October 2014 13:45
To: phenixbb at phenix-online.org
Subject: [phenixbb] anomalous signal

Hello,
I was wondering if the f'' determined by XANES experiment is quantitatively significant and if it can be contributed by two different anomalous scatterers.

For example in my protein I know that Co and Zn are present. In theory, at Zn absorption edge, I have a contribution from Co too. If I perform a XANES experiment to determine Zn absorption edge and I obtain a f'' of 5.87, does this value correspond to only Zn signal or to a sum of Zn+Co?

Thanks a lot

Matteo

--
Matteo Colombo PhD
Groupe Extremophile et grands assemblages moléculaire Institut de Biologie Structurale
71 avenue des Martyrs
CS 10090
38044 Grenoble Cedex 9

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