[phenixbb] Merging reflections
mohamed.noor34 at gmail.com
Mon Jun 1 14:09:11 PDT 2015
I have a few native datasets, one anomalous dataset with signal up to 10 A
and two other datasets (also collected at the phasing wavelength but no
anomalous signal seems to be present) and a sulfur SAD dataset with signal
to about 7 A. All the datasets have a resolution of about 2-3 A and
processed with xia2/XDS.
I couldn't do fluorescence scan as the detector couldn't detect at such a
high energy (20 keV).
A few questions:
1. Why is there at least some signal from one dataset but not others? The
signal should come from a covalently bound ligand, molybdopterin to be
2. Since I(+) should not be equal to I(-) when there is anomalous signal,
should I just merge the native dataset together with those collected at
phasing wavelength that have no anomalous signal? Or do I merge everything
3. Will the anomalous signal to 10 A be useful to do MR-SAD? I could solve
the structure with MR but I wanted to be sure my ligand is there.
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