[phenixbb] anomalous phasing with HySS - phaser - Autobuild
Almudena Ponce Salvatierra
maps.farma at gmail.com
Fri Jun 26 03:32:54 PDT 2015
I have SeMet data for which HySs locates 127 Selenium atoms with a CC of
0.31, which I think is decent.
Then I run Phaser to generate the first maps, and it gives a score of 25
and LLG of 190 or so...
The next step would be running Autobuild, however, the first models I
realize it is building have R work and R free between 50 and 55%. So I
guess it is just some random stuff there.
If the result from HySS and Phaser looks more or less good, why does
AutoBuild show these numbers?? Any idea of what I could do to improve so?
Any ideas are welcome.
Macromolecular crystallography and Nucleic acid chemistry
Max Planck Institute for Biophysical Chemistry
Am Fassberg 11 37077 Göttingen
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