[phenixbb] Molecular replacement at low resolution

Gino Cingolani Gino.Cingolani at jefferson.edu
Sat May 2 11:00:27 PDT 2015

always check k=180 (2-fold ncs) in addition to whatever high symmetry ncs-axis you think is in your data (e.g. k=45, etc).
There's no guarantee it will work. It's an experimental approach to attempt for difficult MR cases.

Gino Cingolani, Ph.D.
Thomas Jefferson University
Dept. of Biochemistry & Molecular Biology
233 South 10th Street - Room 826
Philadelphia PA 19107
Tel: (215) 503 4573
Website:  http://www.cingolanilab.org
"Nati non foste per viver come bruti, ma per seguir virtute e canoscenza"
("You were not born to live like brutes, but to follow virtue and knowledge")
Dante, The Divine Comedy (Inferno,  XXVI, vv. 119-120)

From: phenixbb-bounces at phenix-online.org <phenixbb-bounces at phenix-online.org> on behalf of Engin Özkan <eozkan at uchicago.edu>
Sent: Saturday, May 02, 2015 1:09 PM
To: phenixbb at phenix-online.org
Subject: Re: [phenixbb] Molecular replacement at low resolution

On 5/2/15 11:18 AM, Gino Cingolani wrote:
> If you don't see anything at k=45, then you don't have 8 copies in the
> asu.
While checking self rotation functions is a great idea, I would shy away
from such categorical assertions as self rotation functions are not
always cleanly interpretable (despite GLRF, which is my favorite as
well), and an eight-fold NCS might not be due to an eight-fold rotation:
you can have eight molecules with different arrangements (such as two C4
"tetramers") as well as with improper NCS, or through translational NCS
as well.

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