[phenixbb] Fluorescence group of eYFP

Pavel Afonine pafonine at lbl.gov
Mon Mar 7 08:40:21 PST 2016

Hi Miyatake,

could you please send me (and copy to Nigel) the PDB file (and any 
ligand CIF files you use) and tell what bonds you are expecting to have 
and that brake as result of refinement.


On 3/7/16 03:40, miyatake wrote:
> Dear all,
> I'm trying to a mutant of eYFP, which has fluorescence group,
> CR2.
> During the refinement, however, the bonds between the CR2 and other amino acids
> were cleaved even I added the cif file created by the ReadySet.
> Would some one give me suggestions to solve the problem?
>     Miyatake
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