[phenixbb] FSC curve between experimental cryo EM map and model

[Pavel Afonine]

Hi Tofayel,

phenix.mtriage (analogue of phenix.xtriage) is likely to be available 
some time next week in one of Phenix nightly builds. It will report 
various FSC.

Pavel

On 4/28/17 06:08, #AHMED TOFAYEL# wrote:
>
> Dear all,
>
>
> If I want to calculate FSC curve between experimental cryo EM map and 
> model, what is the correct procedure to do so?
>
>
> I will explain what I have tried so far. Although I have variable 
> local resolution in my map, according to FSC=0.143 criterion I have a 
> 4 Ang map (say 1.mrc), I have used 'molmap' command in Chimera to 
> generate a simulated map at 4 Ang. Then I used "vop resample" to take 
> care of the pixel and box values ( then saved as 2.mrc). Thereafter, I 
> have used "e2proc3d.py 1.mrc fsc.txt --calcfsc=2.mrc and plotted the 
> fsc.txt file to get the curve.
>
>
> I am not sure if I am on the right track. My pixel size is 1.28.
>
>
> Best regards,
>
> Tofayel
>
>

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