[phenixbb] Refine a structure with a disconnected region

Yasser Almeida Hernández yasser.almeida at gmail.com
Thu Feb 2 06:35:46 PST 2017

Hi all,

I am refining a structure at 1.9A, which contains a 7 residues 
C-terminal region. The resolution of the map in that region is low but 
is clear enough to define some side chains and the backbone. I build 
this region manually in Coot. This region is disconnected from the core 
of the protein, since the connecting loop cannot be seen in the density 
map. After running phenix.refine (GUI) and playing with the options 
several times, the final model of this region is always out of the map, 
even when the R values are improved. How can I refine the structure, 
keeping that part of the protein in its place, fitted to the map?

Thanks in advance



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