[phenixbb] sequence file error in phenix map-to-model

Raja Dey rddey80 at gmail.com
Mon Dec 20 10:02:25 PST 2021


Hi,
I am getting an error with the sequence file attached. Could you please
tell me what's wrong with this file? The error is enclosed below:
Starting phenix
on Mon Dec 20 11:45:46 2021 by rdey
===============================================================================

Processing files:
-------------------------------------------------------------------------------

  Found phil,
/atavium/home/jfrench/rdey/st_JF/.phenix/project_data/map_to_model_8.eff

Processing PHIL parameters:
-------------------------------------------------------------------------------

  Adding PHIL files:
  ------------------
    /atavium/home/jfrench/rdey/st_JF/.phenix/project_data/map_to_model_8.eff

Processing files from PHIL:
-------------------------------------------------------------------------------

  Found real_map,
/atavium/home/jfrench/rdey/st_JF/cryosparc_P11_J139_008_volume_map_half_A.mrc

  Files not used by program:
  --------------------------
  /atavium/home/jfrench/rdey/st_JF/D41-st-Trx-sequence.txt
  /atavium/home/jfrench/rdey/st_JF/p11_j140_sharpened-map_box.ncs_spec

Final processed PHIL parameters:
-------------------------------------------------------------------------------
  data_manager {
    real_map_files =
"/atavium/home/jfrench/rdey/st_JF/cryosparc_P11_J139_008_volume_map_half_A.mrc"
    default_real_map =
"/atavium/home/jfrench/rdey/st_JF/cryosparc_P11_J139_008_volume_map_half_A.mrc"
    phil_files =
"/atavium/home/jfrench/rdey/st_JF/.phenix/project_data/map_to_model_8.eff"
    default_phil =
"/atavium/home/jfrench/rdey/st_JF/.phenix/project_data/map_to_model_8.eff"
  }
  input_files {
    map_file =
/atavium/home/jfrench/rdey/st_JF/cryosparc_P11_J139_008_volume_map_half_A.mrc
    seq_file = /atavium/home/jfrench/rdey/st_JF/D41-st-Trx-sequence.txt
    ncs_file =
/atavium/home/jfrench/rdey/st_JF/p11_j140_sharpened-map_box.ncs_spec
  }
  reconstruction_symmetry {
    symmetry = """D2"""
  }
  control {
    nproc = 2
  }
  job_title = """D41-st-Trx-19Dec21"""


Starting job
===============================================================================
Validating inputs


Thanks a lot for your time.
Regards,
Raja
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> HistagTrxTEV
MGSSHHHHHHGTSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQ
NPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGTDYDIPTTENLYFQGHM
> UBA
DVLLSMGFPRARAQKALASTGGRSVQAACDWLFSH
> PDE
VGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTV
SRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPT
LEKLAQNIDVKLGCDWVATIFSRDIRFAN
> SH3
HETLQVIYPYTPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKA
> NoDomain
ENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERRPYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRC 
> HP
LFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESN
TIIDHVYCSPSLRCVQTAHNILKGLQQENHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHI
PISKLVVSESYDTYISRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDF
> DimDomain
VQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE


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