Kim, Doo Nam
doonam.kim at pnnl.gov
Tue Feb 9 12:11:47 PST 2021
Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions?
Yes, this is somehow empirically determined.
I think that my predecessors of cryo_fit used to use 15 A, 10 A, 5 A resolution cryo-EM maps successively to avoid being trapped in local minima when they fit ribosome atomic structure into these.
cascade mdff in https://elifesciences.org/articles/16105
From: <phenixbb-bounces at phenix-online.org> on behalf of Reza Khayat <rkhayat at ccny.cuny.edu>
Date: Tuesday, February 9, 2021 at 11:51 AM
To: "phenixbb at phenix-online.org" <phenixbb at phenix-online.org>
Subject: [phenixbb] Cryo_fit
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The gaussian filter used to prepare cryo-EM maps for cryo_fit is equivalent to a low pass filter. Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions? Thanks.
Reza Khayat, PhD
City College of New York
Department of Chemistry and Biochemistry
New York, NY 10031
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