[phenixbb] Improvement of low resolution MR phases

[Roger Rowlett]

NCS plus jelly-body restraints in Refmac works pretty well for data in the
3.0-3.5A resolution range with multimeric targets. Not a miracle, but more
stable, sensible refinement.

Roger Rowlett


On Mon, Jun 28, 2021, 7:56 AM Kajander, Tommi A <tommi.kajander at helsinki.fi>
wrote:

> Hello all,
>
> I was wondering what would be current best protocols for trying to improve
> maps/phases for low resolution
> MR / refinement solutions (5-6 Å resolution).
>
> High solvent content of course, dimeric 2:2 protein complex so some NCS
> averaging and density modification
> I assume might help (related structures known, currently maps very blobby,
> clear solution but I think should be able to do better...).
>
> Also, refinement suggestions welcome.
>
> Best route in phenix? So far worked initially with ccp4. Other suggestions
> also welcome.
>
> Thanks!
> Tommi
>
>
> Tommi Kajander, Ph.D.
> Institute of Biotechnology
> University of Helsinki
> Viikinkaari 1 (P.O. Box 65)
> 00014 Helsinki, Finland
> p. +358-50-4480991
> http://www.helsinki.fi/kajanderlab
>
>
>
>
>
>
>
>
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