[phenixbb] Double difference anomalous maps

Mark Saper saper at umich.edu
Tue Nov 9 01:08:32 PST 2021


I tried the "Isomorphous difference map” action and specified a file for input 1 containing F1+, F1– at one wavelength, and the file input 2 containing F2+,F2– at another wavelength. I selected the appropriate Labels for the columns containing the anomalous data.  I then specified in "Advanced Options” to use anomalous differences instead of amplitudes.  Will this procedure use the following coefficients for the difference Fourier?

	( F1(+) – F1(–) ) – ( F2(+) – F2(–) ), alpha(calc)

Can someone confirm if this is indeed what is calculated?

Many thanks,
Mark Saper
__________________
Mark A. Saper, Ph.D.		
Visiting Professor at the Faculty of Medicine
Hebrew University of Jerusalem
Ein Kerem Jerusalem
ISRAEL

saper at umich.edu     mobile +972 52 815-0480‬


         
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