<div dir="ltr">Hi all,<br> I have a little problem... R and R-free from phenix.refine are 22.9 and 30.6 respectively.... when i validated in the rcsb server... sfcheck gave me a R and Rfree of 30.8 and 37.8. I am aware that sfcheck never reproduces the result. but i am worried that the difference is quite large... indeed i have used TLS NCS and bulk solvent correction... <br>
<br>Did i do anything wrong... please help me<br><br>Thanks for your help<br><br>regards<br>John<br></div>