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<TITLE>Problem with modified cysteine CME</TITLE>
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<FONT FACE="Verdana, Helvetica, Arial"><SPAN STYLE='font-size:12.0px'>I am trying to refine a structure with modified cysteines, which refmac recognized as CME just fine. Phenix has CME listed in the mon_lib_list.cif, but the CME.cif file was not in the mon_lib/c/ directory. I added it to the phenix mon_lib/c directory but phenix.refine still complained. I next added the CME.cif file ( copied from ccp4) as a command line input to phenix.refine, and added this to my parameters file: <BR>
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refinement.pdb_interpretation.apply_cif_modification {<BR>
data_mod = CME<BR>
residue_selection = chain A and resid 381<BR>
residue_selection = chain B and resid 381<BR>
residue selection = chain B and resid 530<BR>
}<BR>
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Still no luck. <BR>
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Thanks for your help, <BR>
Kendall <BR>
-- <BR>
Kendall W. Nettles, PhD<BR>
Associate Professor<BR>
Department of Cancer Biology<BR>
The Scripps Research Institute<BR>
130 Scripps Way<BR>
Jupiter Fl 33458<BR>
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