<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:14pt"><div><span>Dear All,</span></div><div><span></span> </div><div><span>There may be PDB files which shows different protonation state of HIS, at least there is PDB file shows double protonated HIS. At which resolution of the crystal<var id="yui-ie-cursor"></var> can allow us to distinguish different protonation states of HIS?</span></div><div><span></span> </div><div><span>How do Phenix and Coot give different protonation states of HIS?</span></div><div><span></span> </div><div><span>I am looking forward to getting your reply.</span></div><div><span></span> </div><div><span>Cheers,</span></div><div><span></span> </div><div><span>Dialing</span></div> </div></body></html>