<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:14pt"><div><span>Dear All,</span></div><div><span></span> </div><div><span>By Coot we can build a peptide according to 2Fo-Fc and Fo-Fc. For the most part of the peptide especially in the middle of the peptide, the reliability constructed should be very high. But at each end of the peptide, there are 1-2 residues constructed based on 2Fo-Fc contoured at lower sigma (even 0.8).</span></div><div><span></span> </div><div><span>Will you please explain hot to analysis the reliability of the terminal reisudes constructed at each end of the peptide?</span></div><div><span><var id="yui-ie-cursor"></var></span> </div><div><span>I am looking forward to getting a reply from you.</span></div><div><span></span> </div><div><span>Cheers,</span></div><div><span></span> </div><div><span>Fenghui</span></div>
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