<div dir="ltr"><div class="gmail_extra"><div class="gmail_quote"><div>Dear <span name="Nathaniel Echols">Nathaniel,</span></div><div><span name="Nathaniel Echols"></span> </div><div><span name="Nathaniel Echols">Thanks for your reply. So from your answer, I feel that maybe it's not worth to spend much time to resolve the structure through MR based on current dataset? Even I can correctly place the domainB, it's still hard to resolve the intact protein structure without other phasing information, is that right?</span></div>
<div><span name="Nathaniel Echols"></span> </div><div><span name="Nathaniel Echols">Thanks again and best regards!</span></div><div><span name="Nathaniel Echols"></span> </div><div><span name="Nathaniel Echols">Zhihong</span></div>
<div><span name="Nathaniel Echols"></span> </div><blockquote style="margin:0px 0px 0px 0.8ex;padding-left:1ex;border-left-color:rgb(204,204,204);border-left-width:1px;border-left-style:solid" class="gmail_quote"><div dir="ltr">
<div class="gmail_extra"><div class="gmail_quote"><div class="im"><blockquote style="margin:0px 0px 0px 0.8ex;padding-left:1ex;border-left-color:rgb(204,204,204);border-left-width:1px;border-left-style:solid" class="gmail_quote">
<div dir="ltr"><p style="margin:0in 0in 10pt" class="MsoNormal"> </p></div></blockquote><div><br></div></div><div>I confess I haven't had to solve a structure like this myself - just seen a bunch of datasets where similar attempts didn't work - but it's hard to see how it can be solved without experimental phasing information (SeMet, etc.). The good news is that if you can get a correct MR solution for domainB, you can use MR-SAD to find heavy atom sites and solve the structure, which should be easier than starting from no phases at all.</div>
<div><br></div><div>-Nat</div></div></div></div>
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