<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hi all,<div><br></div><div>Just wondering whether there is any capacity to include DEN restraints in phenix.real_space_refine, e.g. as implemented in the program DireX (see ref below), or any possibility of implementing such in a future release of PHENIX?</div><div><br></div><div>This would be very handy for refinement of models into low resolution maps (particularly EM maps).</div><div><br></div><div>Also, on a different but related topic, does anyone have any suggestions as to how to handle model validation (local and global) at low resolution in real-space? It seems as though there is not a solid consensus on how to handle this.</div><div><br></div><div>Many thanks,</div><div>Oliver.</div><div><span style="text-indent: -16px;"><br></span></div><div><span style="text-indent: -16px;">1.</span><span class="Apple-tab-span" style="text-indent: -16px; white-space: pre;"> </span><span style="text-indent: -16px;">Schr�der, G. F., Br�nger, A. T. & Levitt, M. Combining Efficient Conformational Sampling with a Deformable Elastic Network Model Facilitates Structure Refinement at Low Resolution. </span><i style="text-indent: -16px;">Structure</i><span style="text-indent: -16px;"> </span><b style="text-indent: -16px;">15,</b><span style="text-indent: -16px;"> 1630�1641 (2007).)</span></div></body></html>