<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">I suggest that whoever did the refinement does not yet understand Rfree nor how to keep it free. This can happen if an Rfree set is chosen after the structure or an essentially identical structure is solved.<div class="">For instance, choosing a different Rfree set for a liganded structure or a simple mutant that has already been refined using those spots in Rwork.<br class=""><div class=""><br class=""></div><div class="">G</div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On Nov 20, 2014, at 8:52 AM, Singh, Bishal <<a href="mailto:b.singh@dkfz-heidelberg.de" class="">b.singh@dkfz-heidelberg.de</a>> wrote:</div><br class="Apple-interchange-newline"><div class="">Hello everybody,<br class=""> While depositing a structure in PDB, I observed that Rwork was more than Rfree in the highest resolution shell and in a few more shells as well. I shall be delighted if somebody can suggest me the reasons and also what should I do now. <br class=""><br class="">With regards,<br class="">Bishal<br class="">PhD student,<br class="">DKFZ, University of Heidelberg, Germany<br class="">_______________________________________________<br class="">phenixbb mailing list<br class=""><a href="mailto:phenixbb@phenix-online.org" class="">phenixbb@phenix-online.org</a><br class="">http://phenix-online.org/mailman/listinfo/phenixbb<br class=""><br class=""></div></blockquote></div><br class=""><div apple-content-edited="true" class="">
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