<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:16px"><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr">1- after getting structure refined with phenix.refine to 0.145 , 0.164 Rw/Rf. and then you feel happy with that. </div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr">then after using validation server from PDB you get 0.158 , 0.181 Rw/Rf. why this big jump for R free. why phenix.refine giving us low R free where in reality is not?</div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr"><br></div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr">2- if i want to show kick map for metal binding site for publication, which map and how could i generate it.</div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr"><br></div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr">thank you for your help.</div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr"><br></div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr">HW</div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr">UdeM</div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr" class="" style=""><br></div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr" class="" style=""><br></div><div id="yui_3_16_0_1_1420691632447_454157" dir="ltr" class="" style=""> </div></div></body></html>