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<div class="moz-cite-prefix">Hi Bishal -<br>
<br>
I notice you have something funny happening in the 3.8 - 3.3 A bin
as well. Did you have ice ring problems in your data?<br>
<br>
I suspect your funny R factors are coming from outliers in your
reflection data. Reprocessing the data with a careful eye to
scaling problems or ice rings may help you here. If this doesn't
help, you may need to collect a new dataset...<br>
<br>
Good luck,<br>
Matt<br>
<br>
<br>
On 2/25/15 1:28 PM, Singh, Bishal wrote:<br>
</div>
<blockquote cite="mid:D113D2DE.2592%25b.singh@dkfz-heidelberg.de"
type="cite">
<div>Hello everyone,</div>
<div> I am refining a structure at 2.3 A resolution. R-work/R-free
is 26/31 and is not reducing further. When I looked into the
resolution bin, I notices a sharp rise of R-free between
resolution range 2.5-2.6 A (marked in figure below). However
R-work is fine. I am doubtful of reducing the resolution to 2.5
A or lower. Please suggest me your opinion. Your words will be
highly appreciated.</div>
<div><br>
</div>
<div><img moz-do-not-send="true"
src="cid:D50E01B9-02E4-422D-BDA2-47C533F98358"
type="image/png"></div>
<div><br>
</div>
<div>With regards,</div>
<div>Bishal Singh</div>
<div>PhD Student</div>
<div>University of Heidelberg, Germany</div>
<br>
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<pre class="moz-signature" cols="72">--
Matthew Franklin, Ph. D.
Senior Scientist
New York Structural Biology Center
89 Convent Avenue, New York, NY 10027
(212) 939-0660 ext. 9374</pre>
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