<div dir="ltr"><div><div>Thanks everyone. Pavel, one of the images in your presentation was what I was looking for.<br><br></div>A summary regarding my second question - soaking/exchange can be used if the crystal is large enough and this is cheaper and faster than perdeuteration. The latter method is reserved for small crystals. However, soaking will only exchange labile protons like OH- and not those in CH3 and CH2. One point mentioned off-board was that growing cells in D2O causes enough changes such that cells will grow slower and therefore beta lactam antibiotics like ampicillin is not great.<br><br></div>For those looking for 'special' structures - PDB 4AR3, the really interesting description of hydronium ion (H3O+) in 3KCJ/3QZA/3QYS (Kovalevsky et al, Angewandte Chemie 2011) and ultrahigh resolution structure from neutron data of crambin <span class="">4FC1</span> (thanks Maxime Cuypers and Leif Hanson!!)<br></div><div class="gmail_extra"><br><div class="gmail_quote">On Fri, Apr 17, 2015 at 10:58 PM, mohamed noor <span dir="ltr"><<a href="mailto:mohamed.noor34@gmail.com" target="_blank">mohamed.noor34@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr"><div>Dear all<br><br></div>I will be presenting about protein crystallography to a group of PhD-level physicists and need some images from the community.<br><div><br>Would anyone have high-quality images of density maps that show hydrogen atoms at<br>1. high and ultra-high X-ray resolution (say, about 1.2 A to about 0.5 A)<br>2. low or medium X-ray resolution (where H atoms are not visible) compared with neutron data (with H). Ideally these images should be from the same protein so that I can show the power of complementary beam source for the diffraction.<br><br></div><div>A side question - what is the community's preference to get deuterated samples? Is it crystal soaking or growing E. coli in deuterium?<br></div><div><br></div><div>All contributions will be credited. Thanks.<br></div></div>
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