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Hi Kostya,<br>
<br>
how complete the data set is? Missing reflections in some resolution
zones between 2.64A and inf may be sufficient to make the effective
resolution lower or much lower than 2.64A which would explain why
the map does not look like what you expect.<br>
<br>
Pavel<br>
<br>
<div class="moz-cite-prefix">On 10/4/16 23:47, Kogan, Konstantin
wrote:<br>
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cite="mid:e912a535-c218-afc3-55aa-8d287b9d0589@helsinki.fi"
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<p>Dear all,</p>
<p>I have highly anisotropic data. I was able to solve the
structure by MR, and refine it with phenix.refine to
Rwork/Rfree=0.28/0.31 with Resolution cutoff 2.64� and mean B
values 114, which is pretty high for such resolution. Though the
overall structure makes sense, the maps are quite featureless,
no water molecules, and side-chains refinement is a problem. I
have tested how the data is anisotropic with <a
moz-do-not-send="true"
href="https://services.mbi.ucla.edu/anisoscale/">Diffraction
Anisotropy Server</a> (see attached image), which shows
clearly, that we don't really have data to 2.64� in all
directions. Though, there are other servers/programs that can
deal specifically with anisotropic data e.g. <a
moz-do-not-send="true"
href="http://staraniso.globalphasing.org/cgi-bin/staraniso.cgi">STARANISO
anisotropy server</a> and then refine with BUSTER, I would
like to know if it's possible still to use Phenix with some more
advance parameters to actually address the issue of anisotropy
and to get maximum out of the data. The space group is P61 2 2,
cell parameters are 73, 73, 453, 90, 90, 120 and I have high
multiplicity data, but no NCS.� </p>
Thanks in advance for any input,<br>
<br>
Kostya<br>
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