<html><head></head><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px"><div id="yui_3_16_0_ym19_1_1480614346589_3132">Dear All,</div><div id="yui_3_16_0_ym19_1_1480614346589_3132"> </div><div id="yui_3_16_0_ym19_1_1480614346589_3132">Supposed by phenix.real_space_refine I got a PDB of protein-ligand, will you please let me know how to get the subtraction map (difference map?) which only show the density of the ligand, so that I can see whether the ligand was well resolved in the map, or whether the built ligand fits well with the ligand density?</div><div id="yui_3_16_0_ym19_1_1480614346589_3132"><br></div><div id="yui_3_16_0_ym19_1_1480614346589_3132">Smith</div></div></body></html>