<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class=""><br class=""></div>Hi there,<div class="">I have a pretty basic question.</div><div class="">I am refining a structure in which a drug has been covalently linked to a Cys of the protein.</div><div class="">I have generated the .cif fie for the ligand, but I don’t know how to tell the program that carbon C04 has to be covalently linked to the SG of the Cys. Is that sufficient to insert a LINK record in the pdb file? What is the correct syntax for that?</div><div class="">Thanks a lot for the feedback and sorry for the naive question,</div><div class="">ciao,</div><div class="">Sebastiano</div><div class=""><br class=""></div><div class=""><br class=""></div><div class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div class=""><div class="" style="orphans: 2; text-align: -webkit-auto; widows: 2; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">-- <br class=""><b class="">Sebastiano Pasqualato, PhD</b><br class="">Crystallography Unit<br class="">Department of Experimental Oncology<br class="">European Institute of Oncology<br class="">IFOM-IEO Campus<br class="">via Adamello, 16<br class="">20139 - Milano<br class="">Italy<br class=""><br class="">tel +39 02 9437 5167<br class="">fax +39 02 9437 5990</div><div class="" style="orphans: 2; text-align: -webkit-auto; widows: 2; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">web <a href="http://is.gd/IEOXtalUnit" class="">http://is.gd/IEOXtalUnit</a></div></div></div></div>
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