<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</head>
<body text="#000000" bgcolor="#FFFFFF">
<font size="+1"><tt>Hi Pavel, <br>
<br>
I am very lucky to see 2 maps of the same protein, at roughly
the same resolution, one by Xray and the other by EM (it's not
that common). The details from the 2 maps are however
different... <br>
<br>
Here is my procedure, please let me know what you think: <br>
- convert .mrc map to .mtz ; I get F, PHIF.<br>
- supperpose the maps: <br>
map1: labels 2FOFCWT, PH2FOFCWT<br>
map2: F, PHIF.<br>
I also provided the 2 PDBs as it is clearly stated on the input
files definitions. <br>
<br>
It worked, with the 2 maps superposed that I can look and
compare. <br>
<br>
However, for some reason, the superposition has been done on
chain C and D of the crystallograhic model instead of chain AB
(4 chains in the asymmetric unit, making 2 dimers). Is there a
reason there, choosing the closest structure, best fit? (I see
that it is possible to define a superposition) <br>
Another thing is that it creates a "supperposition in the
middle", meaning neither on model1 nor model2 from the input,
and not a crystallographic symmetric of model1. So I can't use
the full crystallographic maps, I have a "simplified" version of
it. <br>
<br>
Please let me know if I'm doing something wrong as haven't yet
invested into understanding map formats. <br>
Thanks<br>
Vincent <br>
<br>
ps: Zhijie, thanks for the Chimera tip. I'll have a look into it
later. <br>
<br>
</tt></font><br>
<br>
<div class="moz-cite-prefix">On 04/10/2018 12:00, Pavel Afonine
wrote:<br>
</div>
<blockquote type="cite"
cite="mid:a9046644-b920-4ffd-e95a-8600f3c60da9@lbl.gov">Hi
Vincent,
<br>
<br>
<blockquote type="cite">I am trying to superpose a CryoEM map onto
a Xray map.
<br>
I tried the "superpose maps" under the map section of phenix
1.14 but it only takes "reflections" and not the .mrc format of
the CryoEM map.
<br>
should I transform the CryoEM map into structure factors using
"Map to structure factors" or is there a better way to do this?
<br>
</blockquote>
<br>
you can try.. Yes, convert both maps into Fourier space
equivalents and feed into the program. Note, you need two models
that fit two maps. The way it works is that it superposes models
first, then applies corresponding operators (that superpose
models) to maps. I can't remember anyone asking this questions
before on this list or myself directly.
<br>
<br>
Share your experience!
<br>
<br>
Good luck,
<br>
Pavel
<br>
<br>
</blockquote>
<br>
<div class="moz-signature">-- <br>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta http-equiv="Content-Style-Type" content="text/css">
<title></title>
<meta name="Generator" content="Cocoa HTML Writer">
<meta name="CocoaVersion" content="1404.47">
<style type="text/css">
p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; line-height: 14.0px; font: 12.0px Helvetica; color: #000000; -webkit-text-stroke: #000000}
span.s1 {font-kerning: none}
</style>
<p class="p1"><span class="s1">Vincent Chaptal, PhD</span></p>
<p class="p1"><span class="s1">MMSB -UMR5086</span></p>
<p class="p1"><span class="s1">Drug Resistance and Membrane
Proteins Laboratory</span></p>
<p class="p1"><span class="s1">7 passage du Vercors<span
class="Apple-converted-space"> </span></span></p>
<p class="p1"><span class="s1">69007 LYON</span></p>
<p class="p1"><span class="s1">FRANCE</span></p>
<p class="p1"><span class="s1">+33 4 37 65 29 01</span></p>
<p class="p1"><span class="s1"><a class="moz-txt-link-freetext" href="http://mmsb.cnrs.fr/en/">http://mmsb.cnrs.fr/en/</a></span></p>
<p class="p1"><span class="s1"><br>
</span></p>
</div>
</body>
</html>