<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
</head>
<body text="#000000" bgcolor="#FFFFFF">
Hi Filip,<br>
<br>
no, this is not possible in phenix.real_space_refine at the moment.
I feel the pressure by having this sort of questions multiple times
a week, on and off list. So implementing this functionality is in my
todo list. I can't hint you about ETA given I'm booked all the way
till November, sorry. <br>
<br>
A question I ask everyone who requests this feature: why you need to
do this and why cutting out a piece of model and a map in a box
isn't working to mimic this?<br>
<br>
Cheers,<br>
Pavel<br>
<br>
<br>
<div class="moz-cite-prefix">On 7/4/19 17:15, Filip Van Petegem
wrote:<br>
</div>
<blockquote type="cite"
cite="mid:CALUELug_BEoJzWw6AKgn1iL8=Kmf4+UPvMnxAe+hv07+-mkRkg@mail.gmail.com">
<meta http-equiv="content-type" content="text/html; charset=UTF-8">
<div dir="ltr">Dear Phenix users,
<div><br>
</div>
<div>I want to exclude particular regions of a protein model,
docked into a cryo-EM map, from real-space refinement. I know
this is possible for refinement in reciprocal space, but is
this possible for refining a cryo-EM structure against a map?
E.g. I want to totally exclude a region with known crystal
structure, placed in an area of poor local resolution, from
any form of refinement, but want to apply all other options
(morphing, simulated annealing, adp, Global minimization) to
the remainder of the structure. </div>
<div><br>
</div>
<div>(I didn't see this option in
<a
href="https://www.phenix-online.org/documentation/reference/real_space_refine.html"
moz-do-not-send="true">https://www.phenix-online.org/documentation/reference/real_space_refine.html</a> )<br>
</div>
<div><br>
</div>
<div>best regards,</div>
<div><br>
</div>
<div>Filip</div>
<div><br>
</div>
<div> </div>
<div>
<div><br>
</div>
-- <br>
<div dir="ltr" class="gmail_signature"
data-smartmail="gmail_signature">
<div dir="ltr">
<div>Filip Van Petegem, PhD</div>
<div>Professor, Dept. of Biochemistry and Molecular
Biology<br>
The University of British Columbia<br>
2350 Health Sciences Mall - Rm 2.356<br>
Vancouver, V6T 1Z3<br>
<br>
phone: +1 604 827 4267<br>
email: <a href="mailto:filip.vanpetegem@gmail.com"
target="_blank" moz-do-not-send="true">filip.vanpetegem@gmail.com</a><br>
<a href="http://crg.ubc.ca/VanPetegem/" target="_blank"
moz-do-not-send="true">http://crg.ubc.ca/VanPetegem/</a></div>
</div>
</div>
</div>
</div>
<br>
<fieldset class="mimeAttachmentHeader"></fieldset>
<pre class="moz-quote-pre" wrap="">_______________________________________________
phenixbb mailing list
<a class="moz-txt-link-abbreviated" href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a>
<a class="moz-txt-link-freetext" href="http://phenix-online.org/mailman/listinfo/phenixbb">http://phenix-online.org/mailman/listinfo/phenixbb</a>
Unsubscribe: <a class="moz-txt-link-abbreviated" href="mailto:phenixbb-leave@phenix-online.org">phenixbb-leave@phenix-online.org</a></pre>
</blockquote>
<br>
</body>
</html>