<div dir="ltr"><div>Hi Luca,</div><div><br></div>I had similar issue with refinement against a low resolution data. However, choosing input model as reference model for restraints (with little bit of playing around with weight factor) took care of this.<div><br></div><div>Ashu</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Mon, Nov 25, 2019 at 2:12 PM Luca Pellegrini <<a href="mailto:lp212@cam.ac.uk">lp212@cam.ac.uk</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">I have noticed that real-space refinement in cryoEM maps has a tendency to pull long disordered side chains (Lys, Glu etc) back towards main chain density, resulting in unrealistic hairpin-like bent conformations. Is there a way to counter or moderate this behaviour? It would save me from having to manually straighten them all out again afterwards...<br>
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Best wishes,<br>
Luca<br>
<br>
Luca Pellegrini, PhD<br>
Department of Biochemistry<br>
University of Cambridge<br>
Cambridge CB2 1GA<br>
United Kingdom<br>
<br>
<a href="mailto:lp212@cam.ac.uk" target="_blank">lp212@cam.ac.uk</a><br>
tel.: 0044 1223 760469<br>
<a href="https://www.bioc.cam.ac.uk/pellegrini-group/pellegrini-group" rel="noreferrer" target="_blank">https://www.bioc.cam.ac.uk/pellegrini-group/pellegrini-group</a><br>
@PellegriniLab<br>
<br>
<br>
<br>
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