<div dir="ltr"><div>Hi Carlos, differences like this do not seem abnormal. For local resolution, it is common to get a very broad range of values (ie, 2.8-10<span lang="en"><span><span><span>Å), but usually the most frequently occurring value is close to the value provided with full-map methods. It can be tough to visualize with color sometimes, but if you look at the volume histogram of the local resolution map in Chimera you can see the distribution of resolution values. <br></span></span></span></span></div><div><span lang="en"><span><span><span><br></span></span></span></span></div><div><span lang="en"><span><span><span>For subsequent Phenix programs and for data reporting, I suggest using the full map value.<span lang="en"><span><span><span></span></span></span></span></span></span></span></span></div><div><span lang="en"><span><span><span></span></span></span></span></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Mon, Aug 9, 2021 at 1:59 AM Carlos HENRIQUE FERNANDES <<a href="mailto:carlos.henrique_fernandes@sorbonne-universite.fr">carlos.henrique_fernandes@sorbonne-universite.fr</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div><div style="font-family:arial,helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"><div>Dear all,<br></div><div><span lang="en"><span><span>I've been finding some discordance between overall map resolution value obtained at phenix.mtriage (d_FSC) and local map resolution values obtained at phenix.local_resolution.</span></span></span> </div><div><span lang="en"><span><span>phenix.mtriage (using full map and both half maps) provides a d_FSC of 4.31 <span>Å; however, the phenix.local_resolution (using both half maps and not informing the d_FSC) provides local map resolution values have a range of 3.8 - 4.4 Å with most of the map having 4.0 Å resolution with very few parts (only small flexible regions at terminal regions) having 4.3-4.4 Å resolution. </span><br></span></span></span></div><div><span lang="en"><span><span><span>Are these differences normal or is there something wrong with my map refinement? Despite d_FSC being 4.31 Å; can I consider my map at 4.0 Å resolution and do the sharpening and dock in map operations at 4.0 Å? <br></span></span></span></span></div><div><span lang="en"><span><span><span><br></span></span></span></span></div><div><span lang="en"><span><span><span>Thanks in advance,<br></span></span></span></span></div><div><span lang="en"><span><span><span>Carlos Fernandes<br></span></span></span></span></div><div><span lang="en"><span><span><span><br></span></span></span></span></div><div><span lang="en"><span><span><span><br></span></span></span></span></div><div><span lang="en"><span><span><span><br></span></span></span></span></div><div><span lang="en"><span><span><span><span><span></span> </span><span></span></span></span></span></span></div></div></div>_______________________________________________<br>
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