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<p class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
lang="EN-GB">Posted on behalf of Dr. Franck Martin (CNRS
IBMC, Strasbourg, France)</span></i></b></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
lang="EN-GB">Please reply to
<a class="moz-txt-link-abbreviated" href="mailto:F.Martin@ibmc-cnrs.unistra.fr">F.Martin@ibmc-cnrs.unistra.fr</a></span></i></b></p>
<div class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
lang="EN-GB"><br>
</span></i></b></div>
<div class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
lang="EN-GB">=======================================================================================
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<p class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
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<p class="yiv0777537734ydp6915d66aMsoNormal"><b><i><span
lang="EN-GB">URGENT - Recovery Plan Project -
Post-doctoral fellow
(M/F) in molecular and structural biology (Cryo-EM)</span></i></b></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
lang="EN-GB">Mission</span></b><b><span lang="EN-GB"></span></b></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-US">You will be
working as
a post-doctoral researcher in a mixed public/private
environment. The team
'Evolution of translation initiation systems in
eukaryotes' of the unit
'Architecture and Reactivity of RNA' (UPR 9002 of the
CNRS) of the Institute of
Molecular and Cellular Biology (IBMC) of Strasbourg
in association with the company Novalix. NovAliX is a 180+
employees society
based in Illkirch (near Strasbourg).
We offer to the pharmaceutical industry a complete range
of services spanning
biophysics, biochemistry, structural biology, chemistry
& drug discovery. It
is specified that this recruitment offer is part of the
France Recovery
Plan on a research collaboration project with a company
which will be submitted
to the DRARI for validation. The project will consist in
structurally and
functionally characterizing macromolecular complexes
allowing the initiation of
viral RNA of SARS-CoV-2. </span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-US"> </span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
lang="EN-US">Activities</span></b></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">The
approaches used will be based on the use of
cell-free translation extracts that will allow us to
assemble and purify
translation initiation complexes on our model messenger
RNAs. The composition
of these complexes will be determined by mass spectrometry
and their structure
by advanced structural approaches.<span> </span>The
techniques used will be: </span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB"> </span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">-
Biochemical and molecular biology methods dedicated
to the study of proteins and nucleic acids and in
particular RNA</span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">- Cryo-EM
sample preparation, optimizing sample
vitrification conditions.</span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">- Collect
and analyse cryo-EM data. 3D reconstruction,
structure determination and atomic model building.</span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">-
Preparation of in vitro cell-free translation
extracts</span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB"> </span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">The
candidate will have to develop his/her project
independently.<span> </span></span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
lang="EN-GB">Expected
qualifications and proficiencies</span></b></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">This
includes the mastery of biochemical and molecular
biology approaches for the purification of
ribonucleoprotein complexes. Strong
expertise in structural biology
(Crystallography/Cryo-Electron Microscopy)
especially on macromolecular complexes containing the
ribosome will be
considered as a plus. Ideally, the candidate should also
be able to interpret
electron density maps to establish atomic models of
ribonucleic complexes
containing the ribosome. The candidate must have defended
his/her thesis in
2019 or 2020. </span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
lang="EN-GB">Environment</span></b></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">The
candidate will join a biochemistry and molecular
biology team specialising in translation initiation in
eukaryotes. The
structural part of the project will be implemented within
the company Novalix. The
Novalix cryo-EM platform is currently equipped with a
Glacios microscope
outfitted with a phase plate, Falcon 3 camera and microED
setup. The candidate
will spend 20% of his/her time in the public laboratory
for the preparation of
samples and 80% of his/her time at Novalix for the
structural aspect.</span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><span lang="EN-US"> </span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><span lang="EN-US">More on IBMC:
<a class="moz-txt-link-freetext" href="https://ibmc.cnrs.fr/en/">https://ibmc.cnrs.fr/en/</a></span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><span lang="EN-US">More on
Strasbourg:
<a class="moz-txt-link-freetext" href="https://www.visitstrasbourg.fr/en/welcome-in-strasbourg/">https://www.visitstrasbourg.fr/en/welcome-in-strasbourg/</a></span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><span lang="EN-US">More on Alsace
area: <a class="moz-txt-link-freetext" href="https://www.visit.alsace/en/">https://www.visit.alsace/en/</a></span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><span lang="EN-US"> </span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
lang="EN-US">Work constrains and professional risks</span></b></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">The project
will require the manipulation of
radioactivity and the synthesis of RNA.<span>
</span>Therefore, the mastery of good laboratory practices
will be considered a
plus.</span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
lang="EN-GB">Application</span></b></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">Applications
will only be made via the recruitment
website but feel free ton contact Dr. Franck Martin for
any further details
including application rules.</span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">Application
website:</span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB"><a class="moz-txt-link-freetext" href="https://emploi.cnrs.fr/Offres/CDD/UPR9002-FRAMAR-006/Default.aspx?lang=EN">https://emploi.cnrs.fr/Offres/CDD/UPR9002-FRAMAR-006/Default.aspx?lang=EN</a></span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><b><span
lang="EN-GB">Salary</span></b></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"
style="text-align:justify;"><i><span lang="EN-GB">Gross salary
will be between 2675
and 3767 € per month according to working experience.</span></i></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"> </span></p>
<p class="yiv0777537734ydp6915d66aMsoNormal"><span lang="EN-GB"><b>Contact
information</b>: <i><a class="moz-txt-link-abbreviated" href="mailto:F.Martin@ibmc-cnrs.unistra.fr">F.Martin@ibmc-cnrs.unistra.fr</a></i></span></p>
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