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<p>Hi Dmitry,</p>
<p>two options:</p>
<p>1) The model file (PDB or mmCIF) contains symmetry information as
BIOMT records and one asymmetric part of the molecule, then<br>
</p>
<p>phenix.pdb.biomt_reconstruction model.pdb</p>
<p>will generate the whole 14mer.</p>
<p>2) You have the map that covers the whole 14mer and the model
(PDB or mmCIF) that contains only one one asymmetric part:</p>
<p>- Get symmetry operators from the map using phenix.map_symmetry.<br>
- Apply symmetry operators from the previous step to the model
using phenix.apply_ncs to generate 14mer.</p>
<p>All of the command line instructions above can also be run in the
Phenix GUI.</p>
<p>Pavel<br>
</p>
<div class="moz-cite-prefix">On 6/7/23 01:48, Dmitry A. Semchonok
wrote:<br>
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<p class="MsoNormal">Dear colleagues,</p>
<p class="MsoNormal">I want to symmetrise the monomer to
complete the model (14 homo-oligomeric subunits) with D7
symmetry.</p>
<p class="MsoNormal">Is there a tool that can be used to
multiply my monomer with D7 symmetry to fulfil the cryo-EM
model?</p>
<p class="MsoNormal">What are the steps for that?</p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thank you</p>
<p class="MsoNormal">Kind regards,</p>
<p class="MsoNormal">Dmitry</p>
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