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<font size="4"><font face="monospace">Hi Matthias, <br>
<br>
phenix.varref was designed to refine a series of models into a
series of maps that are following each other, such as the ones
coming from 3DVA (cryosparc) or multi-body refinement (Relion).
<br>
It works for very large structures like the spliceosome (2.5
MDa, protein+DNA chains) so it should work for you large model
too. It might be worth testing on your system. <br>
<br>
Varref only runs using the command line for now, and you may
access it through the nightly builds. Dev-4933 is the latest
update so anything stable after that will work. A doc is also
accessible:
<a class="moz-txt-link-freetext" href="https://phenix-online.org/documentation/reference/varref.html">https://phenix-online.org/documentation/reference/varref.html</a><br>
<br>
Don't hesitate to contact me if you need help. <br>
Best<br>
Vincent<br>
</font></font><br>
<div class="moz-cite-prefix">Le 30/09/2023 à 18:27,
Vorländer,Matthias Kopano a écrit :<br>
</div>
<blockquote type="cite"
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<p class="MsoNormal"><span lang="EN-US">Hi everyone, <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Apologies if this has
been asked before, but I would like to know what are the
recommended procedures to refine a model against many
(>10) cryo-EM maps from local refinement? Some of the
maps have substantial overlap between them, and I manually
annotated which part of the model is best resolved in which
map.
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Very grateful for any
suggestions, <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Best wishes,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Matthias<o:p></o:p></span></p>
</div>
<br>
<fieldset class="moz-mime-attachment-header"></fieldset>
<pre class="moz-quote-pre" wrap="">_______________________________________________
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<p class="p1"><span class="s1">Vincent Chaptal, PhD</span></p>
<p class="p1"><span class="s1">Director of GdR APPICOM</span></p>
<p class="p1"><span class="s1">Drug Resistance and Membrane
Proteins Lab</span></p>
<p class="p2"><span class="s1"></span><br>
</p>
<p class="p1"><span class="s1">MMSB -UMR5086</span></p>
<p class="p1"><span class="s1">7 passage du Vercors<span
class="Apple-converted-space"> </span></span></p>
<p class="p1"><span class="s1">69007 LYON</span></p>
<p class="p1"><span class="s1">FRANCE</span></p>
<p class="p1"><span class="s1">+33 4 37 65 29 01</span></p>
<p class="p1"><span class="s1"><a class="moz-txt-link-freetext" href="http://www.appicom.cnrs.fr">http://www.appicom.cnrs.fr</a></span></p>
<p class="p1"><span class="s1"><a class="moz-txt-link-freetext" href="http://mmsb.cnrs.fr/en/">http://mmsb.cnrs.fr/en/</a></span></p>
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