<div dir="ltr"><div dir="ltr">Hi Dmitry,<div>I am guessing that your structure contains C2 symmetry as well as helical symmetry, is that correct? The find_ncs algorithm can do one or the other, but not both at once. For your case I think you want to specify:</div><div><br></div><div> reconstruction_symmetry.symmetry=helical<br></div><div> include_helical_symmetryTrue</div><div><br></div><div>and it should look for helical symmetry only.</div><div>All the best,</div><div>Tom T</div><div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Sun, Oct 22, 2023 at 11:20 AM Dmitry Semchonok <<a href="mailto:semchonok@gmail.com">semchonok@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg8840528578582404041">
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Dear colleagues,
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<div>Please prompt me on correctly applying the symmetry parameters to the helical cryo-em map.
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<div>What I did: </div>
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<li style="list-style-type:"1. ""><span>Found symmetry with Map symmetry. The process finished successfully.
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</span></li><li style="list-style-type:"2. "">Applied the NFC operators. <br>
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<div>But the outcome has only two units (C2 symmetry).</div>
<div>When I put more units in the program setting, nothing happens after the run; moreover, the output is not generated.
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<div> Could you please advise how to fill the helical cryo-EM structure with units?
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<div>Many thanks for considering my request. </div>
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<div>Sincerely, </div>
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<div>Dmitry </div>
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Unsubscribe: <a href="mailto:phenixbb-leave@phenix-online.org" target="_blank">phenixbb-leave@phenix-online.org</a></div></blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div dir="ltr">Thomas C Terwilliger<div>Laboratory Fellow, Los Alamos National Laboratory</div><div>Senior Scientist, New Mexico Consortium</div><div>100 Entrada Dr, Los Alamos, NM 87544</div><div>Email: <a href="mailto:tterwilliger@newmexicoconsortium.org" target="_blank">tterwilliger@newmexicoconsortium.org</a></div><div>Tel: 505-431-0010</div><div><br></div></div></div></div></div></div></div></div></div></div></div>