[phenixbb] anomalous signal
joao.dias at heptares.com
Mon Oct 20 06:48:27 PDT 2014
When you collect data at the Zinc K-edge, you will have a very small component of Co in the f'', since your protein as both Zn and Co.
On the other hand, the f' will still have a significant contribution of both Zn and Co.
I usually check Ethan's website and I am attaching a figure for the Zn, Co absorption edges:
Dr Joao Dias
Heptares Therapeutics Ltd
BioPark, Broadwater Road,
Welwyn Garden City,
Herts, AL7 3AX
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From: phenixbb-bounces at phenix-online.org [mailto:phenixbb-bounces at phenix-online.org] On Behalf Of Matteo Colombo
Sent: 20 October 2014 13:45
To: phenixbb at phenix-online.org
Subject: [phenixbb] anomalous signal
I was wondering if the f'' determined by XANES experiment is quantitatively significant and if it can be contributed by two different anomalous scatterers.
For example in my protein I know that Co and Zn are present. In theory, at Zn absorption edge, I have a contribution from Co too. If I perform a XANES experiment to determine Zn absorption edge and I obtain a f'' of 5.87, does this value correspond to only Zn signal or to a sum of Zn+Co?
Thanks a lot
Matteo Colombo PhD
Groupe Extremophile et grands assemblages moléculaire Institut de Biologie Structurale
71 avenue des Martyrs
38044 Grenoble Cedex 9
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