[phenixbb] a sharp rise of R-free in resolution bin
Matthew Franklin
mfranklin at nysbc.org
Wed Feb 25 12:49:58 PST 2015
Hi Bishal -
I notice you have something funny happening in the 3.8 - 3.3 A bin as
well. Did you have ice ring problems in your data?
I suspect your funny R factors are coming from outliers in your
reflection data. Reprocessing the data with a careful eye to scaling
problems or ice rings may help you here. If this doesn't help, you may
need to collect a new dataset...
Good luck,
Matt
On 2/25/15 1:28 PM, Singh, Bishal wrote:
> Hello everyone,
> I am refining a structure at 2.3 A resolution. R-work/R-free is 26/31
> and is not reducing further. When I looked into the resolution bin, I
> notices a sharp rise of R-free between resolution range 2.5-2.6 A
> (marked in figure below). However R-work is fine. I am doubtful of
> reducing the resolution to 2.5 A or lower. Please suggest me your
> opinion. Your words will be highly appreciated.
>
>
> With regards,
> Bishal Singh
> PhD Student
> University of Heidelberg, Germany
>
>
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--
Matthew Franklin, Ph. D.
Senior Scientist
New York Structural Biology Center
89 Convent Avenue, New York, NY 10027
(212) 939-0660 ext. 9374
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