Hi Munan Shaik,
Phenix autobuild can read several types of sequence files, but the basic
format is very simple: enter one copy of the sequence of each chain,
separated by a blank line or a line starting with ">" between chains.
You can optionally add any lines starting with ">" which will be ignored.
So here is an example:
>>> alpha subunit (type 1)
MTMPYYASAEQIMRDRSELARKGIARGRSVVVLTFRDGVLFVAENPSTALHKVSELYDRL
GFAAVGKYNEFENLRRAGIVHADMRGYSYDRRDVTGRSLANAYAQTLGTIFTEQPKPYEV
EICVAEVGRVGSPKAPQLYRITYDGSIVDEQHFVVMGGTTEPIATAMRESYRADLDLEAA
VGIAVNALRQGGAGEGEKRNVDVASLEVAVLDQSRPRRAFRRIAGTALEQLVPAEPAAAS
ESAPEPKPDTETKPADTQD
>>> beta subunit (type 1)
MTADRPALRTGDRDTRLSFGSNLSSFTDYLRGHAPELLPENRIGHRSHSTRGGDGMESGD
LAPHGTTIVALTYKGGVLLAGDRRATQGNLIASRDVEKVYVTDEYSAAGIAGTAGIAIEL
VRLFAVELEHYEKIEGVPLTFDGKANRLASMVRGNLGAAMQGLAVVPLLVGYDLDADDES
RAGRIVSYDVVGGRYEERAGYHAVGSGSLFAKSALKKIYSPDSDEETALRAAIESLYDAA
DDDSATGGPDLTRGIYPTAVTITQAGAVHVSEETTSELARRIVAERTEQGGSAR
Please let me know if that doesn't do it!
All the best,
Tom T
>> Hi everybody,
>> I have a problem with Phenix autobuild. I want to input sequence file
>> together
>> with the pdb and mtz that I got from molecular replacement. But I have no
>> idea
>> which types of sequence files its required. if anybody please help me out.
>>
>>
>> thanks
>> ===================================================
>> Md. Munan Shaik
>> Department of Chemical Biology
>> University of Padova
>> via G. Colombo 03
>> Padova 35131, Italy
>>
>>
>>
