5 Jan
2010
5 Jan
'10
10:29 p.m.
Hi I am doing refinement using phenix. I did simulated annealing, Rfree goes up to 10% more than before. (40%) and R stays at 35%. If use previous phenix refinment file for same pdb and data , R and Rfree is 25/27%. I thought even different r-free set after simulated annealing and 7 cycles of refinement in phenix should bring Rfree down, but not ? But same data set and model , in CNS, R/Rfree 26/28%. Any suggestions will be appreciated. I am using phenix-dev249 - linux. thanks ram