27 Apr
2026
27 Apr
'26
3:14 p.m.
Dear all, I am trying to refine a high-resolution cryo-EM structure. However, some of the guanine NH2 groups end up out of plane (I used cdl). Is that an expected behavior ? It seems that may be planarity restraints associated with hydrogens are not strong enough. Is there an option to augment them ? May be also the riding option only involves distance and angle restraints ? This is not clear from the manual to me. Also, for OH groups like ribose 2'-OH groups, I assume that the riding option only involves distance restraints and that these H-atoms are otherwise free to rotate Can you please help me out here ? Thanks, Pascal