Hi,
Have you tried to process in P1? That is my first guess when things don't go as expected. Also, you don't have to feed the MR search with the full protein complex model. You can search individual components and check if they sit well in the electron density. If none of this work, I would be more inclined to believe that you might have crystallized a contaminant or a fragment of your protein of interest.
Best wishes
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Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +44 07861 273773
"A sorte acompanha uma mente bem treinada"
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De: Huw Jenkins via phenixbb
On 1 Jun 2026, at 08:51, [email protected] wrote:
I have collected a native dataset that extends to 2.45 Å resolution in space group P2₁2₁2 with unit-cell parameters approximately a = 46.9 Å, b = 91.9 Å, c = 165.0 Å.
Just to check - you have been trying MR in all 8 space groups compatible with P222 point group - i.e. P222, P222(1), P22(1)2, P2(1)22, P2(1)2(1)2, P2(1)22(1), P 22(1)2(1) and P2(1)2(1)2(1)? It's not uncommon for assignment of screw axes based on systematic absences to turn out to be incorrect. Huw _______________________________________________ phenixbb mailing list -- [email protected] To unsubscribe send an email to [email protected] Unsubscribe: phenixbb-leave@%(host_name)s