Hi Tom,
Thanks.
Best wishes,
Reza
________________________________
From: Tom Terwilliger
Sent: 18 November 2023 11:44 AM
To: Reza Khayat
Cc: [email protected]
Subject: [EXTERNAL] Re: [phenixbb] Question about phenix.resolve_cryo_em
Hi Reza,
If you use a model in density modification with resolve_cryo_em, it is necessary to create a multi-model ensemble with refinement in order to reduce model bias. For details on why this works and is necessary, see:
https://scripts.iucr.org/cgi-bin/paper?S205979832001061Xhttps://urldefense.proofpoint.com/v2/url?u=https-3A__scripts.iucr.org_cgi-2Dbin_paper-3FS205979832001061X&d=DwMFaQ&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=JqRoNWgCMUo6tg22Jsy7a8OEgOA1DYAEEue1Jb9bwl0cSZCqeImqLA2xZk3c2xn_&s=j9eKWCQRvD9s6WmS7eS7IlKc5SMw1p3aa4YUJ_pbXzU&e=
All the best,
Tom T
On Fri, Nov 17, 2023 at 4:00 PM Reza Khayat mailto:[email protected]> wrote:
Hi,
Is it possible to turn off the model refinement procedure in phenix.resolve.cryo_em and go straight into density modification? My current model fits reasonably well. Also, Does the routine work well with nucleic acid (e.g. something like RNA from ribosome)?
Best wishes,
Reza
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Thomas C Terwilliger
Laboratory Fellow, Los Alamos National Laboratory
Senior Scientist, New Mexico Consortium
100 Entrada Dr, Los Alamos, NM 87544
Email: [email protected]mailto:[email protected]
Tel: 505-431-0010
