OK Tom, thanks a lot for the very quick reply! I'll try that. But now I'd like to understand - why is the sequence file necessary at all? I thought the local sharpening approach just did the same as the global sharpening, only sliding a small box throughout the volume. It seems then that this is not the case? Best wishes, -- Ricardo Diogo Righetto 2018-01-18 21:53 GMT+01:00 Tom Terwilliger < [email protected]>:
Hi Ricardo,
I'm sorry, yes you do have to supply a sequence file for local sharpening. I will fix the documentation. If you want you can just put in a dummy sequence and supply a value for "solvent_content=xxx" and that will accomplish the same thing.
Let me know if that doesn't do it! all the best, Tom T
On Thu, Jan 18, 2018 at 1:42 PM, Ricardo Righetto < [email protected]> wrote:
Hi,
I am trying to do local sharpening with phenix.auto_sharpen, with the following command:
phenix.auto_sharpen map.mrc resolution=4.0 local_sharpening=True
Then it runs the global sharpening first as expected, but when it gets to the local sharpening it crashes with the following error:
Sorry: Please specify a sequence file with seq_file=myseq.seq
Nowhere it says that I would need a .seq file in order to do local sharpening (global sharpening works fine). Any ideas?
Thanks,
-- Ricardo Diogo Righetto
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-- Thomas C Terwilliger Laboratory Fellow, Los Alamos National Laboratory Senior Scientist, New Mexico Consortium 100 Entrada Dr, Los Alamos, NM 87544 https://maps.google.com/?q=100+Entrada+Dr,+Los+Alamos,+NM+87544&entry=gmail&source=g Email: [email protected] Tel: 505-431-0033