Re: [phenixbb] Cryo_fit

Hi Reza, Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions? ==> Yes, this is somehow empirically determined. I think that my predecessors of cryo_fit used to use 15 A, 10 A, 5 A resolution cryo-EM maps successively to avoid being trapped in local minima when they fit ribosome atomic structure into these. Relevant references: https://www.phenix-online.org/documentation/faqs/cryo_fit2_FAQ.html#how-can-... cascade mdff in https://elifesciences.org/articles/16105 best, Doo Nam From: on behalf of Reza Khayat Date: Tuesday, February 9, 2021 at 11:51 AM To: "[email protected]" Subject: [phenixbb] Cryo_fit Check twice before you click! This email originated from outside PNNL. Hi, The gaussian filter used to prepare cryo-EM maps for cryo_fit is equivalent to a low pass filter. Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions? Thanks. Best wishes, Reza Reza Khayat, PhD Associate Professor City College of New York Department of Chemistry and Biochemistry New York, NY 10031