Phil, the Br is in ligand, as you suggest, I might just see weak anomalous.
Ryan, you are right. I have no evidence to show the Br-ligand I used is in
the protein structure.
resolution does not degrade much along with frames
I think this is just a weak anomalous signal datasets.
Thanks.
Charles
On Thu, Jul 10, 2014 at 1:03 PM, Phil Jeffrey
Charles,
So if you have bound ligand with a covalently-linked Bromine, and the ligand isn't abundant in your unit cell, don't you *expect* the anomalous signal to be weak ? Have you tried a DANO model-phased difference map or something comparable ?
Phil Jeffrey Princeton
On 7/10/14 12:52 PM, CPMAS Chen wrote:
Well, let me make this clear.
1. I am using Br anomalous signal to identify the potentially bound ligand. 2. I do shoot the crystals at 0.92A. 3. the different crystals have different resolution, but anomalous signal was weak as reported by autoxds.
Charles
-- *************************************************** Charles Chen Research Associate University of Pittsburgh School of Medicine Department of Anesthesiology ******************************************************