Hi Dmitry, two options: 1) The model file (PDB or mmCIF) contains symmetry information as BIOMT records and one asymmetric part of the molecule, then phenix.pdb.biomt_reconstruction model.pdb will generate the whole 14mer. 2) You have the map that covers the whole 14mer and the model (PDB or mmCIF) that contains only one one asymmetric part: - Get symmetry operators from the map using phenix.map_symmetry. - Apply symmetry operators from the previous step to the model using phenix.apply_ncs to generate 14mer. All of the command line instructions above can also be run in the Phenix GUI. Pavel On 6/7/23 01:48, Dmitry A. Semchonok wrote:
Dear colleagues,
I want to symmetrise the monomer to complete the model (14 homo-oligomeric subunits) with D7 symmetry.
Is there a tool that can be used to multiply my monomer with D7 symmetry to fulfil the cryo-EM model?
What are the steps for that?
Thank you
Kind regards,
Dmitry