![](https://secure.gravatar.com/avatar/247288304a94fd130cf11bba0111d036.jpg?s=120&d=mm&r=g)
Hi Reza,
Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions?
==>
Yes, this is somehow empirically determined.
I think that my predecessors of cryo_fit used to use 15 A, 10 A, 5 A resolution cryo-EM maps successively to avoid being trapped in local minima when they fit ribosome atomic structure into these.
Relevant references:
https://www.phenix-online.org/documentation/faqs/cryo_fit2_FAQ.html#how-can-...
cascade mdff in https://elifesciences.org/articles/16105
best,
Doo Nam
From:
participants (1)
-
Kim, Doo Nam