Hi Sangeeta, I'm sorry for the problem with phenix.auto_sharpen! Can you possibly try these things that may help figure out the problem:

From the command line, type this:

phenix.version This should tell you your version of phenix, and it will also show that phenix is present in this terminal window. Now type: phenix_regression.list auto_sharpen This will then list a command that you can run on your computer to test auto_sharpen. On mine it looks like: libtbx.python "/net/anaconda/raid1/terwill/misc/PHENIX/modules/phenix_regression/segment_and_split_map/tst_auto_sharpen.py" Copy and paste whatever is printed there and make sure that it runs and ends with "OK". In particular, one of the tests will be: "Testing auto_sharpen with model and remove_aniso" This will be testing your problem case. Let me know (off-list) if this test fails and I will try to help you debug the problem. All the best, Tom T On Wed, Aug 19, 2020 at 1:28 AM sangeeta niranjan wrote:

Hello, I am trying to use Auto Sharpen feature of a CyoEM refined map into Phenix but the job is getting failed every time with the following error: Missing the model file: followed with model file name and location.

Although it was working before but now it's showing the same error for all the auto sharpen runs including Models/PDBs.

Thank you -- Sangeeta Niranjan Ph.D. Student, Lab of structural Biology Lab-6 NB National Centre for cell Science Ganeshkhind, Pune, India

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-- Thomas C Terwilliger Laboratory Fellow, Los Alamos National Laboratory Senior Scientist, New Mexico Consortium 100 Entrada Dr, Los Alamos, NM 87544 Email: [email protected] Tel: 505-431-0010